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large insert human brain cdna library  (TaKaRa)


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    Structured Review

    TaKaRa large insert human brain cdna library
    Large Insert Human Brain Cdna Library, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+brain+large+insert+cdna+library/us07247300-312-5-10
    Average 86 stars, based on 1 article reviews
    large insert human brain cdna library - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Amplification:

    Article Title: Transglutaminase-mediated Intramolecular Cross-linking of Membrane-bound ?-Synuclein Promotes Amyloid Formation in Lewy Bodies
    Article Snippet: .. α-Synuclein cDNA was amplified from a human brain large insert cDNA library (Clontech) and was ligated into pET-3b (Novagen, Darmstadt, Germany). .. K58C/Q99C and K10C/Q99C double mutants were created by site-directed mutagenesis using the QuikChange kit and protocol (Stratagene, La Jolla, CA). α-Synuclein cDNAs were expressed in BL21(DE3) Escherichia coli and purified from the lysates according to Conway et al. ( 18 ), with the exception that HiTrapQ columns (Amersham Biosciences) were used instead of DEAE columns.

    Article Title: Transglutaminase-mediated Intramolecular Cross-linking of Membrane-bound α-Synuclein Promotes Amyloid Formation in Lewy Bodies
    Article Snippet: .. Cloning, Expression, and Purification of Recombinant Proteins— -Synuclein cDNA was amplified from a human brain large insert cDNA library (Clontech) and was ligated into pET-3b (Novagen, Darmstadt, Germany). .. K58C/Q99C and K10C/Q99C double mutants were created by site-directed mutagenesis using the QuikChange kit and protocol (Stratagene, La Jolla, CA).

    Article Title: Transglutaminase-mediated Intramolecular Cross-linking of Membrane-bound ?-Synuclein Promotes Amyloid Formation in Lewy Bodies
    Article Snippet: .. Cloning, Expression, and Purification of Recombinant Proteins α-Synuclein cDNA was amplified from a human brain large insert cDNA library (Clontech) and was ligated into pET-3b (Novagen, Darmstadt, Germany). .. K58C/Q99C and K10C/Q99C double mutants were created by site-directed mutagenesis using the QuikChange kit and protocol (Stratagene, La Jolla, CA). α-Synuclein cDNAs were expressed in BL21(DE3) Escherichia coli and purified from the lysates according to Conway et al. ( 18 ), with the exception that HiTrapQ columns (Amersham Biosciences) were used instead of DEAE columns.

    cDNA Library Assay:

    Article Title: Transglutaminase-mediated Intramolecular Cross-linking of Membrane-bound ?-Synuclein Promotes Amyloid Formation in Lewy Bodies
    Article Snippet: .. α-Synuclein cDNA was amplified from a human brain large insert cDNA library (Clontech) and was ligated into pET-3b (Novagen, Darmstadt, Germany). .. K58C/Q99C and K10C/Q99C double mutants were created by site-directed mutagenesis using the QuikChange kit and protocol (Stratagene, La Jolla, CA). α-Synuclein cDNAs were expressed in BL21(DE3) Escherichia coli and purified from the lysates according to Conway et al. ( 18 ), with the exception that HiTrapQ columns (Amersham Biosciences) were used instead of DEAE columns.

    Article Title: Transglutaminase-mediated Intramolecular Cross-linking of Membrane-bound α-Synuclein Promotes Amyloid Formation in Lewy Bodies
    Article Snippet: .. Cloning, Expression, and Purification of Recombinant Proteins— -Synuclein cDNA was amplified from a human brain large insert cDNA library (Clontech) and was ligated into pET-3b (Novagen, Darmstadt, Germany). .. K58C/Q99C and K10C/Q99C double mutants were created by site-directed mutagenesis using the QuikChange kit and protocol (Stratagene, La Jolla, CA).

    Article Title: Transglutaminase-mediated Intramolecular Cross-linking of Membrane-bound ?-Synuclein Promotes Amyloid Formation in Lewy Bodies
    Article Snippet: .. Cloning, Expression, and Purification of Recombinant Proteins α-Synuclein cDNA was amplified from a human brain large insert cDNA library (Clontech) and was ligated into pET-3b (Novagen, Darmstadt, Germany). .. K58C/Q99C and K10C/Q99C double mutants were created by site-directed mutagenesis using the QuikChange kit and protocol (Stratagene, La Jolla, CA). α-Synuclein cDNAs were expressed in BL21(DE3) Escherichia coli and purified from the lysates according to Conway et al. ( 18 ), with the exception that HiTrapQ columns (Amersham Biosciences) were used instead of DEAE columns.

    Cloning:

    Article Title: Transglutaminase-mediated Intramolecular Cross-linking of Membrane-bound α-Synuclein Promotes Amyloid Formation in Lewy Bodies
    Article Snippet: .. Cloning, Expression, and Purification of Recombinant Proteins— -Synuclein cDNA was amplified from a human brain large insert cDNA library (Clontech) and was ligated into pET-3b (Novagen, Darmstadt, Germany). .. K58C/Q99C and K10C/Q99C double mutants were created by site-directed mutagenesis using the QuikChange kit and protocol (Stratagene, La Jolla, CA).

    Article Title: Transglutaminase-mediated Intramolecular Cross-linking of Membrane-bound ?-Synuclein Promotes Amyloid Formation in Lewy Bodies
    Article Snippet: .. Cloning, Expression, and Purification of Recombinant Proteins α-Synuclein cDNA was amplified from a human brain large insert cDNA library (Clontech) and was ligated into pET-3b (Novagen, Darmstadt, Germany). .. K58C/Q99C and K10C/Q99C double mutants were created by site-directed mutagenesis using the QuikChange kit and protocol (Stratagene, La Jolla, CA). α-Synuclein cDNAs were expressed in BL21(DE3) Escherichia coli and purified from the lysates according to Conway et al. ( 18 ), with the exception that HiTrapQ columns (Amersham Biosciences) were used instead of DEAE columns.

    Expressing:

    Article Title: Transglutaminase-mediated Intramolecular Cross-linking of Membrane-bound α-Synuclein Promotes Amyloid Formation in Lewy Bodies
    Article Snippet: .. Cloning, Expression, and Purification of Recombinant Proteins— -Synuclein cDNA was amplified from a human brain large insert cDNA library (Clontech) and was ligated into pET-3b (Novagen, Darmstadt, Germany). .. K58C/Q99C and K10C/Q99C double mutants were created by site-directed mutagenesis using the QuikChange kit and protocol (Stratagene, La Jolla, CA).

    Article Title: Transglutaminase-mediated Intramolecular Cross-linking of Membrane-bound ?-Synuclein Promotes Amyloid Formation in Lewy Bodies
    Article Snippet: .. Cloning, Expression, and Purification of Recombinant Proteins α-Synuclein cDNA was amplified from a human brain large insert cDNA library (Clontech) and was ligated into pET-3b (Novagen, Darmstadt, Germany). .. K58C/Q99C and K10C/Q99C double mutants were created by site-directed mutagenesis using the QuikChange kit and protocol (Stratagene, La Jolla, CA). α-Synuclein cDNAs were expressed in BL21(DE3) Escherichia coli and purified from the lysates according to Conway et al. ( 18 ), with the exception that HiTrapQ columns (Amersham Biosciences) were used instead of DEAE columns.

    Purification:

    Article Title: Transglutaminase-mediated Intramolecular Cross-linking of Membrane-bound α-Synuclein Promotes Amyloid Formation in Lewy Bodies
    Article Snippet: .. Cloning, Expression, and Purification of Recombinant Proteins— -Synuclein cDNA was amplified from a human brain large insert cDNA library (Clontech) and was ligated into pET-3b (Novagen, Darmstadt, Germany). .. K58C/Q99C and K10C/Q99C double mutants were created by site-directed mutagenesis using the QuikChange kit and protocol (Stratagene, La Jolla, CA).

    Article Title: Transglutaminase-mediated Intramolecular Cross-linking of Membrane-bound ?-Synuclein Promotes Amyloid Formation in Lewy Bodies
    Article Snippet: .. Cloning, Expression, and Purification of Recombinant Proteins α-Synuclein cDNA was amplified from a human brain large insert cDNA library (Clontech) and was ligated into pET-3b (Novagen, Darmstadt, Germany). .. K58C/Q99C and K10C/Q99C double mutants were created by site-directed mutagenesis using the QuikChange kit and protocol (Stratagene, La Jolla, CA). α-Synuclein cDNAs were expressed in BL21(DE3) Escherichia coli and purified from the lysates according to Conway et al. ( 18 ), with the exception that HiTrapQ columns (Amersham Biosciences) were used instead of DEAE columns.

    Recombinant:

    Article Title: Transglutaminase-mediated Intramolecular Cross-linking of Membrane-bound α-Synuclein Promotes Amyloid Formation in Lewy Bodies
    Article Snippet: .. Cloning, Expression, and Purification of Recombinant Proteins— -Synuclein cDNA was amplified from a human brain large insert cDNA library (Clontech) and was ligated into pET-3b (Novagen, Darmstadt, Germany). .. K58C/Q99C and K10C/Q99C double mutants were created by site-directed mutagenesis using the QuikChange kit and protocol (Stratagene, La Jolla, CA).

    Article Title: Transglutaminase-mediated Intramolecular Cross-linking of Membrane-bound ?-Synuclein Promotes Amyloid Formation in Lewy Bodies
    Article Snippet: .. Cloning, Expression, and Purification of Recombinant Proteins α-Synuclein cDNA was amplified from a human brain large insert cDNA library (Clontech) and was ligated into pET-3b (Novagen, Darmstadt, Germany). .. K58C/Q99C and K10C/Q99C double mutants were created by site-directed mutagenesis using the QuikChange kit and protocol (Stratagene, La Jolla, CA). α-Synuclein cDNAs were expressed in BL21(DE3) Escherichia coli and purified from the lysates according to Conway et al. ( 18 ), with the exception that HiTrapQ columns (Amersham Biosciences) were used instead of DEAE columns.



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    Upregulation of septin 3 isoforms during RA-induced neural differentiation of SH-SY5Y cells. SH-SY5Y cells were treated for 4 days with vehicle (–) or 2.5 μM RA (+). (a) <t>cDNA</t> synthesized from mRNA of treated cells was used as template for PCR. Three sets of primers were used to detect mRNAs for septin <t>3A,</t> <t>3B,</t> and GAPDH as an internal control. The expected sizes of septin 3A (top), 3B (middle), and GAPDH (bottom) mRNAs were 1160, 1308, and 1000 bp, respectively. (b) Lysates from treated cells were separated by 10% SDS-PAGE and then subjected to Western blotting (WB) with antibodies against septin 3A (top), 3B (middle), or β-actin as an internal loading control (bottom).
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    Image Search Results


    Upregulation of septin 3 isoforms during RA-induced neural differentiation of SH-SY5Y cells. SH-SY5Y cells were treated for 4 days with vehicle (–) or 2.5 μM RA (+). (a) cDNA synthesized from mRNA of treated cells was used as template for PCR. Three sets of primers were used to detect mRNAs for septin 3A, 3B, and GAPDH as an internal control. The expected sizes of septin 3A (top), 3B (middle), and GAPDH (bottom) mRNAs were 1160, 1308, and 1000 bp, respectively. (b) Lysates from treated cells were separated by 10% SDS-PAGE and then subjected to Western blotting (WB) with antibodies against septin 3A (top), 3B (middle), or β-actin as an internal loading control (bottom).

    Journal: Gene Expression

    Article Title: Expression of Septin 3 Isoforms in Human Brain

    doi:

    Figure Lengend Snippet: Upregulation of septin 3 isoforms during RA-induced neural differentiation of SH-SY5Y cells. SH-SY5Y cells were treated for 4 days with vehicle (–) or 2.5 μM RA (+). (a) cDNA synthesized from mRNA of treated cells was used as template for PCR. Three sets of primers were used to detect mRNAs for septin 3A, 3B, and GAPDH as an internal control. The expected sizes of septin 3A (top), 3B (middle), and GAPDH (bottom) mRNAs were 1160, 1308, and 1000 bp, respectively. (b) Lysates from treated cells were separated by 10% SDS-PAGE and then subjected to Western blotting (WB) with antibodies against septin 3A (top), 3B (middle), or β-actin as an internal loading control (bottom).

    Article Snippet: The human cDNA fragments of septin 3A, 3B, and Nedd5 were produced by polymerase chain reaction (PCR) from the Human Fetal Brain Large-Insert cDNA library (BD Biosciences Clontech, Palo Alto, CA).

    Techniques: Synthesized, SDS Page, Western Blot